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  Sculpt a Sequence
  Design Golden Gate Overhangs
  Simulate Golden Gate
  Simulate multi-method assemblies
  Insert Parts on Backbones
  Sketch Constructs
  Convert Sequence Files
  Domesticate Part Batches
  Transfer Genbank Features
  Substitute Parts Overhangs
  Design Sites Arrays
  Plot Sequence Features
  Evaluate Manufacturability
  Find Common DNA Blocks
  Compare Two Sequences
  Render Sequenticons
  View Overhangs Crosstalk
  Predict Bad Clone Rates
  Extract Features
  Predict Digestions
  Select digestions
  Analyze Digests
  Select primers
  Find Saboteur Parts
  Design Part Test Batches
  Create Assembly Picklists

View Overhangs Crosstalk

View overhang crosstalk for your Golden Gate assembly project.

About this app

This app downloads experimental data from this BioRxiv paper, Optimization of Golden Gate assembly through application of ligation sequence-dependent fidelity and bias profiling, Potapov et al., 2018.

It reads the spreadsheet provided in supplementary information, keeps only the data relevant to your overhangs, and plots it as a heatmap, similar to the figures shown in the paper.

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