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  Sculpt a Sequence
  Design Golden Gate Overhangs
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  Transfer Genbank Features
  Substitute Parts Overhangs
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Substitute Parts Overhangs

Use this app to easily change the position (a.k.a "slot") of parts in Golden-Gate like assembly standards.

Provide parts to modify

Examples

example_sequences.zip

 

Genbank records of five parts (A, A2, B, B2, C) and receptor vector.

Drop a file here or click to upload

Accepted formats: FASTA, Genbank, Snapgene

Use file names as IDs.

Select an enzyme

BsaI

BsmBI

BbsI

Autoselect

Substitutions

These substitutions will only affect overhangs, no changes will occur elsewhere in the part sequence.

Return a FASTA of linear parts for ordering.

Substitute overhangs

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