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  Select digestions
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Select Primers

Find optimal Sanger sequencing plans for your constructs, maximizing primer reuse and minimizing the total numbers of reads and of new primers to buy.

Validation type

• Sanger Sequencing
• Validation PCR (NOT IMPLEMENTED YET)

Strands covered

• Both
• 3'-5'
• 5'-3'
• Any

Constructs Sequences

The sequences should be Genbanks with features annotated "cover" for the zones to be covered (it can be one big zone covering the full sequence) or "no_primer" to forbid primers in certain zones.

Examples

20_random_constructs.zip

 

Collection of constructs assembled using random parts from the EMMA standard. Unzip the file and drag the genbank files into the file upload area.

Drop a file here or click to upload

Fasta or Genbank files

Sequences are circular

Available primers

Examples

available_primers.fa

 

Collection of constructs assembled using random parts from the EMMA standard. Unzip the file and drag the genbank files into the file upload area.

Drop a file here or click to upload

Primers properties

Ideal read range: from 150bp after primer annealing to 800bp

Primer melting temperatures from 55C to 70C

Digits in new primers names:

Select primers

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