Find sets of compatible overhangs for Golden Gate assembly, to extend an assembly standard or decompose a sequence into compatible fragments
A 50kb sequence of phage lambda. Tsuge et al. managed to assemble this sequence in a one-step assembly of fifty 1kb fragments, digested by type-IIs enzymes. This required a very careful selection of enzyme overhangs.
A 50kb sequence of phage lambda. Tsuge et al. managed to assemble this sequence in a one-step assembly of fifty 1kb fragments, digested by type-IIs enzymes. This required a very careful selection of enzyme overhangs.
Number of fragments:
Add sequences (e.g. enzyme sites) at the end of each fragment ?
Differences between overhangs:
Overhangs GC content
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