Design Golden Gate Overhangs

Find sets of compatible overhangs for Golden Gate assembly, to extend an assembly standard or decompose a sequence into compatible fragments

What do you wish to do ?

Sequence

Examples
Drop a file here or click to upload
Fasta or genbank. No file too large please :)

    Sequence decomposition

    Number of fragments:

    Add sequences (e.g. enzyme sites) at the end of each fragment ?

    Overhangs parameters

    Differences between overhangs:

    Overhangs GC content

    25% to 75%:

    Forbidden overhangs

    External overhangs