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  Sculpt a Sequence
  Design Golden Gate Overhangs
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  Simulate multi-method assemblies
  Insert Parts on Backbones
  Sketch Constructs
  Convert Sequence Files
  Domesticate Part Batches
  Transfer Genbank Features
  Substitute Parts Overhangs
  Design Sites Arrays
  Plot Sequence Features
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  Find Common DNA Blocks
  Compare Two Sequences
  Render Sequenticons
  View Overhangs Crosstalk
  Predict Bad Clone Rates
  Extract Features
  Predict Digestions
  Select digestions
  Analyze Digests
  Select primers
  Find Saboteur Parts
  Design Part Test Batches
  Create Assembly Picklists

Create Assembly Picklists

Provide a source plate map and an assembly plan, get a robotic picklist spreadsheet for Tecan EVO or Labcyte Echo.

Assembly Plan

Examples

example_picklist.xls

 

Picklist to build 7 assemblies using common parts of the EMMA standard

Drop a file here or click to upload

Excel file or CSV

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Dispenser

Machine

• Tecan EVO
• Labcyte ECHO

Min volume (nL)

Max one-time volume (µL)

Volume resolution (nL)

Dead volume (µL)

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Mix

Part quantity unit

• femto-mole
• nano-gram
• nano-molar

Part quantity (fmol)

Buffer volume (µL)

Total volume (µL)

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Parts infos

Since a molar quantity is desired, we need to know the molecular weigth of the DNA. Provide either

• A table of the parts sizes, backbone included (see example file) or
• A series of Genbank/Fasta sequences of the parts.

Examples

emma_parts.zip

 

ZIP archive containing the sequences of standard EMMA parts.

emma_parts_sizes.csv

 

Spreadsheet indicating the total size of EMMA parts (including vector backbone).

Drop a file here or click to upload

Genbank/Fasta/Snapgene sequences, or spreadsheet

Use file names (not record IDs) to identify parts.

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Source Plate

Examples

example_echo_plate.xlsx

 

Map of a plate with common genetic parts from the EMMA standard.

Drop a file here or click to upload

Fasta or Genbank files

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Destination Plate

Plate

• New Plate
• Existing Plate

Plate size

• 96 wells
• 384 wells
• 1536 wells

Fill by

• Row
• Column

Generate a picklist

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