Provide the results of an automated restriction digestion, and get a complete
report comparing your results to the expected patterns.
Analysis
Sequence validation
Sequence identification
Constructs Sequences
The title of each Genbank file (or Fasta entry) must be the construct's name.
Examples
constructs_sequences.zip
Collection of constructs assembled using random parts from the EMMA standard.
Unzip the file and drag the genbank files into the file upload area.
Drop a file here or click to upload
All sequences are circular
All sequences are linear
Autodetect each sequence's topology (default to circular)
Autodetect each sequence's topology (default to linear)
Constructs Map
A spreadsheet where the cells contents indicate the construct in each well
Examples
constructs_map.xlsx
Collection of constructs assembled using random parts from the EMMA standard.
Unzip the file and drag the genbank files into the file upload area.
Drop a file here or click to upload
Digestions
AatII
Acc65I
AccI
AclI
AcuI
AflII
AgeI
ApaI
ApaLI
ApoI
AseI
AvaI
BaeGI
BamHI
BanI
BanII
BbsI
BclI
BfuAI
BglII
BmgBI
BmtI
BpuEI
BsaAI
BsaHI
BsaI
BsaJI
BsiEI
BsiHKAI
BsiWI
BsmI
BsoBI
Bsp1286I
BspDI
BspEI
BspHI
BsrBI
BsrGI
BssHII
BstBI
BstYI
BtgI
ClaI
DraI
EagI
EarI
Eco53kI
EcoRI
EcoRV
FspI
HaeII
HincII
HindIII
HpaI
Hpy166II
Hpy188III
KpnI
MfeI
MluI
MspA1I
NaeI
NarI
NcoI
NdeI
NspI
NgoMIV
NheI
NotI
NruI
NsiI
PaeR7I
PluTI
PmlI
PspOMI
PstI
PvuI
PvuII
SacI
SacII
SalI
ScaI
SfoI
SmaI
SmlI
SnaBI
SpeI
SphI
SspI
StuI
StyI
XbaI
XhoI
XmaI
Fragment Analysis ZIP File
Provide a ZIP file of the AATI fragment analyzer (only machine supported at the moment)
Examples
fragment_analyzer_data.zip
Fragment analysis data from the digestion (DraI+HindIII) of constructs
assembled using random parts from the EMMA standard. The results are
bad enough to be totally unpublishable so here they are.
Drop a file here or click to upload
Validation parameters
Tolerance
Distance at which two bands are considered different (in proportion of the ladder span)